Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet: MSM-DTM adsorbs inflammatory cytokines, suppresses TSPCs inflammation and senescence, and promotes tenogenic differentiation and migration (A) The remaining concentrations of TNF-α, IL-1β, IL-6, IFN-γ, LPS, and NGF were detected by ELISA after co-culture with different concentrations of MSM. (B and C) RT-qPCR of IL-6 and CCL-2 in TSPCs under different treatments. (D) Western blot of COL1A2, MMP3, and TNMD. (E) ELISA of TNF-α, IL-6, and IFN-γ in supernatants. (F)Western blot of p16 and p21 after 7 days of treatment. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H and I) Immunofluorescence and quantification of TNMD and TNC after 14-day treatment. Scale bars, 50 μm. (J) Relative mRNA expression of tenogenic differentiation markers SCX and TNC. (K and L) Representative images and quantification of Transwell assay. Scale bars, 100 μm. (M and N) Representative images and quantification of wound healing assay. Scale bars, 100 μm. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups.

Article Snippet: NGF ELISA kit , ABclonal , RK10077.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Quantitative RT-PCR, Western Blot, Staining, Immunofluorescence, Expressing, Transwell Assay, Wound Healing Assay

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet:

Article Snippet: NGF ELISA kit , ABclonal , RK10077.

Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet: MSM-DTM adsorbs inflammatory cytokines, suppresses TSPCs inflammation and senescence, and promotes tenogenic differentiation and migration (A) The remaining concentrations of TNF-α, IL-1β, IL-6, IFN-γ, LPS, and NGF were detected by ELISA after co-culture with different concentrations of MSM. (B and C) RT-qPCR of IL-6 and CCL-2 in TSPCs under different treatments. (D) Western blot of COL1A2, MMP3, and TNMD. (E) ELISA of TNF-α, IL-6, and IFN-γ in supernatants. (F)Western blot of p16 and p21 after 7 days of treatment. (G) β-Galactosidase staining analysis after 7 days of treatment. Scale bars, 100 μm. (H and I) Immunofluorescence and quantification of TNMD and TNC after 14-day treatment. Scale bars, 50 μm. (J) Relative mRNA expression of tenogenic differentiation markers SCX and TNC. (K and L) Representative images and quantification of Transwell assay. Scale bars, 100 μm. (M and N) Representative images and quantification of wound healing assay. Scale bars, 100 μm. Statistical comparisons were performed with one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as the mean ± SD, n = 3. Ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 between groups.

Article Snippet: The remaining cytokines in the supernatant were quantified using ELISA kits for TNF-α (ABclonal, China), IL-1β (ABclonal, China), IL-6 (ABclonal, China), IFN-γ (ABclonal, China), and NGF (ABclonal, China), and LPS (ABclonal, China), following the manufacturers’ protocols.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Quantitative RT-PCR, Western Blot, Staining, Immunofluorescence, Expressing, Transwell Assay, Wound Healing Assay

Journal: Cell Reports Medicine

Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

doi: 10.1016/j.xcrm.2026.102937

Figure Lengend Snippet:

Article Snippet: The remaining cytokines in the supernatant were quantified using ELISA kits for TNF-α (ABclonal, China), IL-1β (ABclonal, China), IL-6 (ABclonal, China), IFN-γ (ABclonal, China), and NGF (ABclonal, China), and LPS (ABclonal, China), following the manufacturers’ protocols.

Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

Journal: bioRxiv

Article Title: Schwann cell p75NTR sustains persistent pain downstream to NGF through ROS-dependent TRPA1 signaling

doi: 10.64898/2026.08.10.743924

Figure Lengend Snippet: (a) Schematic representation of the pain behaviors (acute nociception, mechanical allodynia, heat hyperalgesia, and cold hypersensitivity) evaluated following intraplantar (i.pl./10 µl) proNGF administration. (b) Dose-dependent acute nociception and dose- and time-dependent mechanical allodynia, heat hyperalgesia and cold hypersensitivity after proNGF (0.1, 0.5, 1 µg/10 µl, i.pl.) or vehicle (Veh) in C57BL/6J mice. Schematic representation of LM11A-31 targeting p75NTR receptor in (c) proNGF and (e) NGF-induced pain behaviors. (d) mechanical allodynia and cold hypersensitivity after i.pl. proNGF (1 µg) or Veh in C57BL/6J pretreated with LM11A-31 (100 nmol) or Veh. (f) acute nociception, mechanical allodynia, heat hyperalgesia and cold hypersensitivity after i.pl. NGF (1 µg) or Veh in C57BL/6J pretreated with LM11A-31 (100 nmol) or Veh. (n=6 mice per group). Data are mean ± s.e.m. 1-way or 2-way ANOVA, Bonferroni correction. *P<0.05, ***P<0.001, ****P<0.0001 vs. Veh, ## P<0.01, #### P<0.0001 vs. NGF/Veh, proNGF/Veh.

Article Snippet: Recombinant nerve growth factor (NGF, #N-130) and cleavage-resistant proNGF (#N-255) were from Alomone Labs (Jerusalem, Israel).

Techniques:

Journal: bioRxiv

Article Title: Schwann cell p75NTR sustains persistent pain downstream to NGF through ROS-dependent TRPA1 signaling

doi: 10.64898/2026.08.10.743924

Figure Lengend Snippet: (a) Schematic representation of NGF-induced pain behaviors (acute nociception, mechanical allodynia, heat hyperalgesia, and cold hypersensitivity) in P1p-Cre mice infected with AAV for selective silencing of p75NTR ( Plp-Ngfr mice) in Schwann cells. (b) acute nociception, mechanical allodynia, heat hyperalgesia and cold hypersensitivity after intraplantar (i.pl./10 µl) injection of NGF (1 µg) or vehicle (Veh) in Plp-Ngfr or Control mice. (c) Schematic representation of proNGF-induced pain behaviors in Plp-Ngfr mice. (d) mechanical allodynia and cold hypersensitivity after i.pl. NGF (1 µg) or Veh in Plp-Ngfr or Control mice. ( e) Schematic representation of NGF-induced pain behaviors in Adv-Cre mice infected with AAV for selective silencing of TrkA ( Adv- Ntrk1 mice) in primary sensory neurons. (f) acute nociception, mechanical allodynia, heat hyperalgesia and cold hypersensitivity after after i.pl. NGF (1 µg) or Veh in Adv-Ntrk1 or Control mice. (g) Schematic representation of proNGF-induced pain behaviors in Adv Ntrk1 mice. (h) mechanical allodynia and cold hypersensitivity after i.pl. proNGF (1 µg) or Veh in Adv-Ntrk1 or Control mice. (n=6 mice per group). Data are mean ± s.e.m. 1-way or 2-way ANOVA, Bonferroni correction. *P<0.05, **P<0.01, ****P<0.0001 vs. Control/Veh ## P<0.01, #### P<0.0001 vs. Control/NGF and Control/proNGF.

Article Snippet: Recombinant nerve growth factor (NGF, #N-130) and cleavage-resistant proNGF (#N-255) were from Alomone Labs (Jerusalem, Israel).

Techniques: Infection, Injection, Control

Journal: bioRxiv

Article Title: Schwann cell p75NTR sustains persistent pain downstream to NGF through ROS-dependent TRPA1 signaling

doi: 10.64898/2026.08.10.743924

Figure Lengend Snippet: (a) Schematic representation of ROS inhibition by N-tert-butyl-α-phenylnitrone (PBN) in NGF-induced pain behaviors (acute nociception, mechanical allodynia, heat hyperalgesia, and cold hypersensitivity). (b) acute nociception, mechanical allodynia, heat hyperalgesia and cold hypersensitivity after intraplantar (i.pl./10 µl) NGF (1 µg) or vehicle (Veh) in C57BL/6J mice pretreated with PBN (670 nmol) or Veh. ( c) Schematic representation of ROS inhibition by PBN in proNGF-induced pain behaviors. (d) Mechanical allodynia and cold hypersensitivity after i.pl. proNGF (1 µg) or Veh in C57BL/6J mice pretreated with PBN (670 nmol) or Veh. (n=6 mice per group). Concentration response curve of Ca 2+ evoked by (e) NGF or (g) proNGF in human Schwann Cells (SCs) (cells number: NGF: 10 nM=238, 100 nM=179, 1µM=203, 10µM=196, 100µM=127; proNGF: 10 nM=77, 100 nM=87, 1µM=92, 10µM=106, 100µM=87). Typical traces and pooled data of Ca 2+ responses in SCs after (f) NGF (4 µM) or (h) proNGF (2 µM) in presence of thapsigargin (2 µM), Ca 2+ free medium, LM11A-31 (1 µM), GW441756 (GW, 1 µM), A-967079 (A96, 30 µM) or Veh (0.001% DMSO) (cells number: NGF: Veh=131, NGF/Veh=196, NGF/Thapsigargin=159, NGF/ Ca 2+ free=122, NGF/ LM11A-31 =99, NGF/GW=93, NGF/A96=127; proNGF: Veh=103, proNGF/Veh=106, proNGF/Thapsigargin=104, proNGF/Ca 2+ free=100, proNGF/LM11A-31=76, proNGF/GW441756=64, proNGF/A96=99). (i) Typical traces and cumulative data (area under the curve, AUC) of MitoSOX Red fluorescence in SCs stimulated with (i) NGF (4 µM) or (j) proNGF (2 µM) in the presence of LM11A-31 (1 µM), GW (1 µM), Mito TEMPO (5 µM) or Veh (0.001% DMSO) (cells number: NGF: Veh=74, NGF/Veh=88, NGF/LM=76, NGF/GW=87, NGF/MitoTEMPO=80; proNGF: Veh=70, proNGF/Veh=107, proNGF/LM=75, proNGF/GW=105, proNGF/MitoTEMPO=75). Data are mean ± s.e.m. 1-way or 2-way ANOVA, Bonferroni correction. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. Veh/PBN ### P<0.001, #### P<0.0001 vs. NGF/Veh and proNGF/Veh.

Article Snippet: Recombinant nerve growth factor (NGF, #N-130) and cleavage-resistant proNGF (#N-255) were from Alomone Labs (Jerusalem, Israel).

Techniques: Inhibition, Concentration Assay, Fluorescence

Journal: bioRxiv

Article Title: Schwann cell p75NTR sustains persistent pain downstream to NGF through ROS-dependent TRPA1 signaling

doi: 10.64898/2026.08.10.743924

Figure Lengend Snippet: Typical traces and pooled data of Ca 2+ responses in human Schwann Cells (SCs) after (a) NGF (4 µM) or (b) proNGF (2 µM) in presence of Ca 2+ free medium, A967079 (A96, 30µM) or vehicle (Veh, 0.001% DMSO) (cells number: NGF: Veh=34, NGF/Veh=91, NGF/Ca 2+ free=88, NGF/A96=70, proNGF: Veh=104, proNGF/Veh=62, proNGF/Ca 2+ free=87, proNGF/A96=98). Typical traces and pooled data of Hyper 7 fluorescence after (c) NGF (4 µM) or (d) proNGF (2 µM) in presence of LM11A-31 (1 µM) ML-171 (1 µM), MitoTEMPO (5 µM), A96 (30 µM) or Veh (0.001% DMSO) (cells number: NGF: Veh=51, NGF/Veh=116, NGF/LM=94, NGF/ML-171=55, NGF/MitoTEMPO=75, NGF/A96=114; proNGF: Veh=40, proNGF/Veh=72, proNGF/ LM11A-31 =84, proNGF/ML-171=81, proNGF/MitoTEMPO=101, proNGF/A96=66). Data are mean ± s.e.m. 1-way ANOVA, Bonferroni correction. (e) Schematic representation of the signaling cascade induced by NGF- and proNGF-mediated p75NTR activation in Schwann cells. (1) NGF and proNGF activate p75NTR in Schwann cells, leading to (2) Ca²⁺ release from intracellular stores and (3) superoxide dismutase- ( SOD)-dependent superoxide (O₂⁻) generation. (4) Reactive oxygen species activate TRPA1, resulting in extracellular Ca²⁺ influx and (5) Ca²⁺-dependent activation of NADPH oxidase 1 (NOX1). (6) NOX1-derived hydrogen peroxide (H₂O₂) sustains a feed-forward mechanism by further activating TRPA1 in Schwann cells and, simultaneously, ( 7) activates neuronal TRPA1 to signal pain.

Article Snippet: Recombinant nerve growth factor (NGF, #N-130) and cleavage-resistant proNGF (#N-255) were from Alomone Labs (Jerusalem, Israel).

Techniques: Fluorescence, Activation Assay, Derivative Assay

Journal: bioRxiv

Article Title: Schwann cell p75NTR sustains persistent pain downstream to NGF through ROS-dependent TRPA1 signaling

doi: 10.64898/2026.08.10.743924

Figure Lengend Snippet: (a) Schematic representation of TRPA1 inhibition by A-967079 in NGF-induced pain behaviors (acute nociception, mechanical allodynia, heat hyperalgesia, and cold hypersensitivity). (b) acute nociception, mechanical allodynia, heat hyperalgesia and cold hypersensitivity after intraplantar (i.pl./10 µl) NGF (1 µg) or vehicle (Veh) in C57BL/6J mice pretreated with A-967079 (A96, 300 nmol, i.pl.) or Veh. (c) Schematic representation of TRPA1 inhibition by A-967079 in proNGF- induced pain behaviors. (d) mechanical allodynia and cold hypersensitivity after i.pl. proNGF (1 µg) or Veh in C57BL/6J mice pretreated with A96 (300 nmol, i.pl.) or Veh. (e) Schematic representation of NGF-induced pain behaviors in P1p-Trpa1 fl/fl mice. (f) acute nociception, mechanical allodynia, heat hyperalgesia and cold hypersensitivity after i.pl. NGF (1 µg) or Veh in in P1p-Trpa1 fl/fl or Control mice. (g) Schematic representation of proNGF-induced pain behaviors in P1p-Trpa1 fl/fl mice. (h) mechanical allodynia and cold hypersensitivity after i.pl. proNGF (1 µg) or Veh in in P1p-Trpa1 fl/fl or control mice. ( i,j ) H 2 O 2 content in sciatic nerve tissue homogenates of P1p-Trpa1 fl/fl or Control mice after i.pl. NGF (1 µg) or veh (i) and proNGF (1 µg) or Veh (j). (n=6 mice per group). Data are mean ± s.e.m. 1-way or 2-way ANOVA, Bonferroni correction. *P<0.05, ***P<0.001, ****P<0.0001 vs. Veh/A96, Control/Veh, # P<0.05, ## P<0.01, ### P<0.001, #### P<0.0001 vs NGF/Veh, ProNGF/Veh, Control/NGF, Control/ProNGF.

Article Snippet: Recombinant nerve growth factor (NGF, #N-130) and cleavage-resistant proNGF (#N-255) were from Alomone Labs (Jerusalem, Israel).

Techniques: Inhibition, Control, Tissue

Journal: CNS Neuroscience & Therapeutics

Article Title: hUC ‐ MSCs via β‐ NGF Alleviate Cognitive Impairment After Tibial Fracture Surgery by Regulating the STMN2 / NMNAT2 ‐ SARM1 ‐ NF ‐ κB Signaling Pathway

doi: 10.1002/cns.71062

Figure Lengend Snippet: hUC‐MSCs exert therapeutic effects in the PND mouse model via β‐NGF. (A) 20‐Human Neuro Discovery array analysis between blank‐medium and MSC‐conditioned medium (MSC‐CM) (B) ELISA quantification of β‐NGF levels in blank‐medium and MSC‐CM ( n = 3). (C, D) Representative Western blot bands and quantitative analysis of β‐NGF protein expression in hippocampal tissues from CON, CON‐MSC, SUR, and SUR‐MSC groups. (E) Total distance traveled by mice in OFT ( n = 9). (F) Alternative corrections (%) in Y‐maze test ( n = 9). (G) Novel object preference (%) in NOR ( n = 9). (H) Representative GFAP, NeuN and IBA1 immunostaining images of hippocampal CA1 region. (I) Representative Golgi staining images (J) Quantitative analysis of NeuN positive area percentage in hippocampal CA1 region ( n = 6). (K) Quantitative analysis of GFAP positive area percentage in hippocampal CA1 region ( n = 6). (L) Quantitative analysis of IBA1 positive area percentage in hippocampal CA1 region ( n = 6). (M) Quantitative analysis of dendritic spine density in hippocampal CA1 region. Representative Western blot bands (N) and quantitative analysis of β‐NGF (O), p‐TrkA/TrkA (P), STMN2 (Q) and NMNAT2 (R) protein expression in hippocampal tissues from CON, SUR, SUR‐MSC NC and SUR‐MSC NGFsiRNA groups ( n = 6). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The supernatant was harvested following the manufacturer's instructions, and the concentrations of IL‐1β (HUABIO, China, EM0001), IL‐6 (LIANKE, China, EK206), TNF‐α (HUABIO, China, EM0010), mouse (HUABIO, China, RK00154), and human β‐NGF (Abclonal, China, RK03064) were quantified using ELISA kits.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Immunostaining, Staining